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Ym155 (Sepantronium Bromide)

YM155, Sepantronium

Survivin (BIRC5) transcription inhibitor / IAP inhibitor

Evidence Score

31

preclinical
Mechanism of Action

Ym155 (sepantronium bromide) suppresses transcription of BIRC5 (survivin) by displacing the transcription factor Sp1 from the CDE/CHR elements in the BIRC5 promoter, reducing survivin mRNA and protein levels. The mechanistic rationale for selectivity in SDH-deficient tumors rests on two converging lines. First, SDH loss drives pseudohypoxic HIF-1α stabilization (via succinate-mediated PHD inhibition), and the BIRC5 promoter contains canonical HRE elements through which HIF-1α transcriptionally upregulates survivin above the already-elevated baseline found in most cancers. Second, SDH-deficient cells accumulate unrepaired DNA double-strand breaks via the KDM4B/H3K9me3 BRCAness mechanism (Mechanism 14). In wild-type cells, such DNA damage triggers caspase-mediated apoptosis; in SDH-deficient cells, HIF-1α-driven survivin elevation suppresses caspase-3/7 and forms a ternary anti-apoptotic complex with XIAP and caspase-9, allowing cells to tolerate an otherwise lethal DNA damage load. Ym155 targets both functions simultaneously: depleting survivin removes both the apoptosis block and the CPC/spindle checkpoint support on which genomically unstable SDH-deficient cells depend for mitotic fidelity. Direct SDH-deficient evidence: PMID 41711310 (Endocr Relat Cancer 2026) demonstrated that SDH-deficient cancer cells show significantly increased susceptibility to Ym155-induced DNA damage versus SDH-intact controls — a selectivity that is consistent with the dual BRCAness/Survivin-dependency model. Clinical data: Phase 2 data in relapsed/refractory non-Hodgkin lymphoma (NCT00390117) showed partial responses with survivin suppression confirmed pharmacodynamically; dose-limiting toxicity includes renal impairment at higher doses, manageable with adequate hydration. Key limitations: (1) no dedicated SDH-deficient tumor trial exists; (2) the specific degree of HIF-1α-driven BIRC5 upregulation in SDH-deficient GIST versus PPGL models has not been measured; (3) Ym155 showed limited single-agent activity in Phase 2 solid tumor studies (NCT00437957), suggesting combination strategies or patient selection by BIRC5 expression/HIF-1α level may be required.

Pathway Connections
Succinate-Driven Homologous Recombination Deficiency

Succinate accumulation competitively inhibits the α-KG-dependent histone demethylases KDM4A and KDM4B (JMJD2A/B), which normally erase repressive H3K9me3 marks at sites of DNA double-strand breaks. When KDM4B is inhibited, H3K9me3 hypermethylation persists at break sites, blocking recruitment of TIP60 acetyltransferase and ATM kinase — both required for DNA end-resection and initiation of homology-directed repair (HDR/HR). The result is a 'BRCAness' phenotype: SDH-deficient tumor cells have impaired HR capacity despite wild-type BRCA1/2. Sulkowski et al. (Nat Genet 2018, PMID: 30013182) directly demonstrated HR deficiency and olaparib hypersensitivity in cells and tumors from SDH-deficient hereditary paraganglioma/PPGL patients; Sulkowski et al. (Nature 2020, PMID: 32494005) dissected the KDM4B/H3K9me3 chromatin mechanism.

Upstream event:

SDH loss → succinate accumulation → competitive inhibition of KDM4A/KDM4B (α-KG-dependent H3K9me3 demethylases) → H3K9me3 persistence at DNA double-strand break sites → impaired TIP60/ATM recruitment → defective DNA end-resection → HR deficiency

Downstream effects:

H3K9me3 hypermethylation at DNA double-strand break sitesImpaired TIP60 acetyltransferase and ATM kinase recruitmentDefective homologous recombination (BRCAness phenotype in BRCA1/2-wild-type cells)PARP inhibitor synthetic lethality (trapping unrepaired single-strand breaks in HR-deficient background)Selective sensitivity to olaparib and other PARP inhibitors in SDH-deficient versus SDH-intact cells
HIF-1α-Driven Apoptosis Evasion (Survivin / BIRC5)

Pseudohypoxic HIF-1α stabilization — a universal consequence of SDH loss — transcriptionally activates BIRC5 (survivin), an inhibitor of apoptosis (IAP) family protein. The survivin promoter contains canonical hypoxia-response elements (HREs) directly bound by HIF-1α. Elevated survivin in SDH-deficient cells enables two pro-tumor functions: (1) apoptosis evasion by inhibiting caspase-3/7 and forming a ternary anti-apoptotic complex with XIAP and caspase-9, protecting cells from executing the apoptosis that would normally follow accumulated DNA damage; and (2) mitotic survival as a core subunit of the Chromosomal Passenger Complex (CPC), which governs spindle assembly checkpoint and chromosomal segregation in genomically unstable cells. SDH-deficient cells accumulate DNA damage via BRCAness (Mechanism 14) but evade apoptosis through elevated Survivin — creating a synthetic lethal dependency on BIRC5 that can be exploited by Survivin inhibitors. Direct evidence: PMID 41711310 demonstrated selective susceptibility of SDH-deficient cancer cells to the Survivin inhibitor Ym155 (Endocr Relat Cancer 2026).

Upstream event:

SDH loss → succinate accumulation → PHD inhibition → HIF-1α stabilization → HRE-driven BIRC5/Survivin transcriptional upregulation

Downstream effects:

Elevated BIRC5/Survivin protein in SDH-deficient tumor cellsCaspase-3/7 inhibition — apoptosis evasion despite DNA damage accumulationXIAP-Survivin-caspase-9 ternary complex preventing apoptotic cascade initiationCPC-mediated mitotic survival in genomically unstable cellsSynthetic lethality with Survivin inhibitors (Ym155) in SDH-deficient cells
Molecular Targets

BIRC5

Baculoviral IAP repeat-containing protein 5 (Survivin)

synthetic_lethal

IAP (inhibitor of apoptosis) family protein encoded by BIRC5. Survivin inhibits caspase-3/7-mediated apoptotic execution and serves as a core subunit of the Chromosomal Passenger Complex (CPC) governing mitotic fidelity. Its promoter contains canonical hypoxia-response elements (HREs) directly activated by HIF-1α, making BIRC5 a transcriptional target of the pseudohypoxic program that is constitutively active in all SDH-deficient tumors. SDH-deficient cells with elevated Survivin depend on BIRC5 to evade apoptosis despite accumulated DNA damage from BRCAness; BIRC5 knockdown or inhibition with Ym155 shows selective susceptibility in SDH-deficient cancer cell lines (PMID 41711310).

UniProt: O15392

Quick Facts

Tumor Type Applicability

All SDH tumors
Not FDA Approved
ChEMBL IDCHEMBL1213296
PubChem CID9908089
Clinical Trials
Evidence

Evidence from PubMed, OpenTargets, and ChEMBL will appear here once external data integration is enabled.

Coming in Phase 3

For research exploration only — not medical advice. Consult your doctor before acting on any information.

AI Analysis

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